Tag
His-Tag

Part:BBa_K2834002:Design

Designed by: Viana Isabel Pérez Domínguez   Group: iGEM18_Tec-Chihuahua   (2018-10-07)


6x His-Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The silent mutations were necessary in order to fulfill IDT's synthesis requirements.


Source

The sequence was based on another part (BBa_K2342003) but it was modified by adding a stop codon and some silent mutations.

References